Animals were randomly divided in 5 groups which each group consisted of 6 male mice. Group 1: control (receiving no medication), group 2: diabetic group receiving streptozotocin at a dose of 65 mg / kg intraperitoneally, group 3: received streptozotocin at 65 mg/kg and after confirmation of diabetes, they received calycosin at 60 mg / kg intraperitoneally in 4 weeks, group 5: received calycosin-loaded nanoliposomes at 60 mg / kg intraperitoneally in 4 weeks, group 5: The positive control group was diabetic mice that received 100 mg /kg of vitamin E for 4 weeks. After the treatment period, the animals were anesthetized with ether, then the kidney tissue was removed, washed with mannitol buffer, and homogenized with normal PBS by homogenizer. The homogenized tissue is first centrifuged (10,000 rpm, 10 min) and the supernatant was then discarded and the sediment containing mitochondria was dispersed in Tris buffer on ice [38].

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