A protocol to assess maximal oxygen uptake (V̇O2max) will be performed to test the hypothesis that aerobic exercise will enhance aerobic fitness as measured by V̇O2max and that the V̇O2max improvement due to training will be maintained over 1 year after completion of the exercise intervention. The V̇O2max test will be conducted on a treadmill and will involve a ramp increase in workload aimed at reaching the subject’s V̇O2max within 8–12 min, according to ACSM recommendations [64]. Exercise testing will be completed in the Clinical and Translational Exercise Physiology Laboratory, Cumming School of Medicine, University of Calgary by certified exercise physiologists (Canadian Society of Exercise Physiology). The experimental set-up and the protocol used are similar to those described in previous studies from our lab (see [65]). Outcome measures for this test include V̇O2max, ventilatory thresholds, heart rate, and blood pressure.

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