Statistical analysis was performed using SPSS 21.0 software (IBM Corp.) and data are expressed as the mean ± SEM of at least three independent experiments. Statistical differences between groups were determined using a one-way ANOVA followed by a Tukey's post hoc test. A paired Student's t-test was used to analyze the mRNA expression levels of PFKFB2 between NSCLC and adjacent normal tissues. P<0.05 was considered to indicate a statistically significant difference.

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