Eighty-eight C57BL/6 mice (12-week-old) of both sexes were purchased from Shandong skobas Biotechnology Co., Ltd. (Shandong, China). Mice were housed in the Laboratory Animal Center of Nanjing University of Chinese medicine under specific pathogen-free (SPF) and maintained at 27 °C under a 12-h light/dark cycle with 50% of humidity throughout the experiments. Experiments performed in this study were all approved by the Animal Experiment Committee of Nanjing University of Chinese Medicine (ethics No:201904A009).

Eight mice served as control animals (Blank B), and the others were randomly divided into two groups, namely the model group (n = 40) and the sham group (n = 40). Each group was further divided into five subgroups depending on when the animals were killed (n = 8). We established a surgically induced moderate OA model by anterior cruciate ligament transection surgery (ACLT) which was described in previous reseaches [7, 15], and the groups were named as M1, M2, M4, M8, M12 (1, 2, 4, 8, 12 weeks after ACLT). In the sham group, only a 1.5-cm incision in the same position was made without cutting the ligament, and then the groups were named as S1, S2, S4, S8, S12 (1, 2, 4, 8, 12 weeks after sham surgery).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.