The dissolution studies were performed based on the previous study [37]. Briefly, the dissolution medium (pH 7.4, PBS, 900 ml) under stirring (100 rpm) was added by calycosin-loaded nanoliposomes which is equivalent to 50 mg of drug and at different time intervals, 15 mL samples were removed, replaced, filtrated (0.45 m), and dissolved in mixed solvent (methanol: distilled water = 5.5:3.5, v/v). Finally, 25 µl of the sample was injected into a HPLC system. The rest of the setup was similar to the above-mentioned explanations.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.