Body composition was performed prior to placement in indirect calorimetry cages and again before euthanasia using the EchoMRI-900 instrument (EchoMRI, Houston, TX, USA). Mice were weighed by hand before entering indirect calorimetry cages, at injection times, before diet changes, and before euthanasia. Fat-free mass was calculated by subtracting fat mass from body mass. Percent fat and percent fat-free mass were calculated by dividing fat or fat-free mass by body mass. Change in fat and fat-free mass was calculated by subtracting the value obtained at the start of the experiment from the value obtained before euthanasia.

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