A wound healing method was used to evaluate the migration of ccRCC cells. Briefly, when the cells grew to confluence, the cells were treated with serum free medium for 24 h. Then a scratch on the cell monolayer was made with a sterile pipette tip. The detached cells were removed by washing with PBS and then the cells were maintained in serum-free RPMI-1640 medium. At regular time, the images of the culture were captured, the un-healing area of each scratch was measured and the wound healing ratio was calculated. The experiments were performed in triplicate.

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