According to the manufacturer’s protocol, total cellular RNA was isolated with TRIzol® reagent ( 9109, Thermo Fisher Scientific, MA, USA.) and cDNA was generated using a PrimeScript TM RT Master Mix kit ( RR01AM, Takara Biotechnology, Dalian, China). A fragment of cDNA was amplified by using the following primers: KDF1 forward, 5’-GTACCCAGCAAGCCATGA-3’ and KDF1 reverse, 5’-CTCCCAGAAAGGGTGTGG-3’.

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