SPSS 19.0 software (IBM, Corp.) was used for data processing. Normally distributed data are presented as mean ± SD from three independent experiments. One-way ANOVA was used to compare multiple groups. When the variances were homogeneous, the Tukey's test was used for post hoc analysis. When the variances were uneven, Tamhane's T2 test was used for post hoc analysis. An unpaired Student's t-test was used to compare two groups. P<0.05 was considered to indicate a statistically significant difference. Experiments was repeated for three times.

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