CRC-associated miRNAs were identified in the dbDEMC 2.0 (21) and miRCancer (22) databases. These databases were also used to find the tripartite overlapping data of differentially expressed miRNAs and miRNAs associated with CRC using Biovenn (23). The target genes of differentially expressed miRNAs were predicted by the PicTar (24), Targetscan (25) and miRanda (26) databases. The cut-off value determined using mirSVR was −0.49. The regulatory network of miR-142-3p and its targeted genes were visualized using Cytoscape (27). Tripartite overlapping genes were identified, and enriched pathways and cellular functions were identified by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses (david.ncifcrf.gov/tools.jsp) using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) v6.8 (28).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.