Cells in the logarithmic growth stage were resuspended, counted, inoculated in a 24-well culture plate (~105 cells, depending on the size of the cells) and cultured at 37°C in a 5% CO2 incubator until the degree of cell fusion reached ~60%. PGL3 plasmid (Shanghai GeneChem Co., Ltd.) containing firefly and adrenal luciferase reporter genes was transfected into cells at room temperature using the X-tremeGENE HP transfection reagent (cat. no. 06366236001; Roche Diagnostics) for 24–48 h to observe the fluorescent marker gene expression on the plasmid and to determine the transfection efficiency, or cells were transfected for 48 h to detect luciferase activity using a Dual-Luciferase® Reporter Assay system (cat. no. E2910; Promega Corporation), according to the manufacturer's instructions (1921).

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