Aliquots of 2×105 exponentially growing mimic- and inhibitor-transfected cells were seeded into separate 6-well plates and cultured under standard conditions until they reached 100% confluency. Subsequently, a scratch was made on each plate using a P10 pipette tip. The culture medium was replaced with fresh serum-free DMEM and the cells were cultured further. Images of the wound were obtained at 0 and 24 h using an inverted microscope (magnification, ×200; Olympus Corporation) and quantitated using ImageJ version 1.8.0 (National Institutes of Health).

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