Aliquots containing 2×105 BEAS-2B cells were seeded in each well of 6-well plates and cultured for 24 h. Then, the cells were transfected with: i) 50 nM mimic of miR-200b (UAAUACUGCCUGGUAAUGAUGA); ii) mimic negative control (NC, UUCUCCGAACGUGUCA); iii) 100 nM inhibitor of miR-200b (UCAUCAUUACCAGGCGGUAUUA); or iv) 5-carboxyfluorescein (FAM) labeled inhibitor NC (CAGUACUUUUGUGUAGUACAA). The four oligos were obtained from Guangzhou RiboBio Co., Ltd. Transfection was performed using Lipofectamine® 6000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Transfection efficiency was evaluated by reverse transcription-quantitative (RT-q)PCR and fluorescence of FAM-inhibitor NC transfected cells. The cells were harvested 24 h after transfection and used for wound healing and Transwell assays, as well as for RNA and protein extraction, as described below.

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