K562 cells were purchased from The Cell Bank of Type Culture Collection of Chinese Academy of Sciences and were maintained in RPMI-1640 medium (Hyclone; Cytiva) supplemented with 10% FBS (Zhejiang Tianhang Biotechnology Co., Ltd.) and 1% penicillin/streptomycin solution (Beijing Solarbio Science & Technology Co., Ltd.), at 37°C and 5% CO2. Lentivirus vectors (LV-GATA1, cat. no. 26211-1; LV-GATA1-RNAi, cat. no. 18817-1; LV-hsa-mir-210, cat. no. 41113-2; LV-hsa- miR-210-3p-inhibition, cat. no. 5212-1; LV-SMAD2-RNAi, cat. no. 15901-1) and transfection reagent (HitransG A&P, cat. no. REVG003-1) were purchased from Shanghai GeneChem Co., Ltd.

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