Baicalin (molecular weight, 446.36; purity, >95.4%; batch no. 110715-201821; Fig. 1A) and azithromycin (batch no. 130609-201706) were purchased from National Institutes for Food and Drug Control in China. Baicalin and azithromycin were dissolved in ddH2O, prepared as a 1 mg/ml solution and stored at 4°C. Standard MP FH (ATCC 15531) was purchased from American Type Culture Collection, was dissolved in complete PPLO broth medium (BD Biosciences), mixed thoroughly and incubated at 37°C. MP in the logarithmic growth phase was quantified by color change unit (CCU) and was adjusted to the required concentration using DMEM containing 10% fetal bovine serum (both from Gibco; Thermo Fisher Scientific, Inc.) (34).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.