Functional annotation of all the identified wheat proteins was performed using HMMER 3.2 (June 2018) by comparison of the protein sequences with the HMM PFAM A database 32.0 (February 2019; p < 0.01, dom e-value < 0.01). The complete results of this analysis are provided in Supplementary Table 2. Only the best result has been kept, and the correspondence between the Gene Ontology (GO) ID and PFAM ID was performed using PFAM2GO mapping (released April 21, 2018) (Mitchell et al., 2015). All GO terms matching the identified wheat proteins can be found in Supplementary Table 3. Gene Ontology enrichment in the different protein clusters was computed using a chi-squared test between the observed and expected protein lists as described in Fabre et al. (2019a). Adjusted p values were calculated with the FDR procedure for multiple testing under dependency (Benjamini and Yekutieli, 2001) and deemed significant when < 0.01. Wheat sequences were also compared with the plant susceptibility factors already described and experimentally verified in the literature using a BLAST analysis (best hit p < 1 × 10–80, identity > 70%).

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