In this study, IHC staining of the transplanted tumor tissues was performed using the IHC staining step 1 on the Leica Bond-Max/Bond-III automatic immunohistochemical staining machine. Antigen was repaired using the heat-induced epitope repair (HIER) method. The primary antibody: Anti-Ki67 (ab16667) and Anti-p21(ab188224) and secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) were purchased from Abcam.

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