Libraries were prepared from double-stranded cDNA produced from mRNA that was isolated from the tissue samples by the INCPM (Nancy and Stephen Grand Israel National Center for Personalized Medicine, Rehovot, Israel) RNA-seq unit (Weizmann Institute of Science, Rehovot, Israel) as described previously [57]. Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of 20 samples per lane. Between 22 and 26 million single-end 60-bp reads were sequenced per sample on an Illumina HiSeq 2500 V4 instrument.

Bioinformatics analyses were carried out at the Bioinformatics Core Facility at the Ben-Gurion University (Beer-Sheva, Israel) as described previously [57]. Human- and murine-specific, uniquely mapped genes were analyzed. Differentially expressed genes were defined as those having a p-value < 0.05, and a linear fold change >1.5 and <−1.5. Functional analysis was performed using the Gene Ontology system, DAVID, and Expander software tools.

Functional classification of differentially expressed genes to GO-Slim Biological Processes was performed using Panther [58].

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