The activity of SOD was assessed using nitro tetrazolium blue reduction method as previously described [29]. The activity of CAT was assessed using hydrogen peroxide substrate, which forms a yellowish complex with molybdate upon decomposition [30]. The activity of GPx was assessed following a method that is based on glutathione oxidation by hydrogen peroxide [31]. The activity of GR was determined using a method that is based on glutathione disulfide reduction in the presence of NADPH, which is later oxidized to NADP+ [32]. The activity of GST was assessed using a method based on GSH conjugation with 1-chloro-2,4-dinitrobenzene as substrate [33]. Total GSH level was determined using a method based on 5-thio-2-nitrobenzoic acid reduction. In this assay, 5,5-dithiobis-2-nitrobenzoic acid reacts with the sulfhydryl group of GSH to form 5-thio-2-nitrobenzoic acid [34].

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