The left kidney was used for histopathological study. Briefly, the kidney was fixed in 10% formalin for 48 h, excised and processed with an automated tissue processor (Leica, Nussloch, Germany), before embedding in blocks of paraffin. Four µm thick sections were used for H & E and PAS staining. Thereafter, the sections were observed under a light microscope (Olympus BX41, Tokyo, Japan for assessment on the presence of glomerulosclerosis and thickening of the medullary collecting ducts at 400× magnification. PAS-stained sections were analyzed using ImageJ software (ImageJ, NIH-Bethesda, MD, USA) for staining intensity.

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