PBECs were plated into fibronectin/collagen-coated filter inserts of 12 mm diameter (Costar-Corning Transwell Clear, pore size: 0.4 µm). After reaching confluency, endothelial monolayers were supplied with 550 nM hydrocortisone, 250 μM CPT-cAMP and 17.5 μM RO-201724 and placed into the CellZscope instrument (nanoAnalytics, Münster, Germany). TEER was followed continuously. Treatments were applied after approx. 24 h, when TEER reached plateau. TEER was further monitored for 48 h.

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