Porcine brain endothelial cells (PBECs) were isolated from the grey matter of young female Vietnamese pot-bellied pigs, as described previously [37], selected with 4 µg/mL puromycin (Merck-Sigma, Darmstadt, Germany) for 4 days and cultured in Dulbecco’s modified Eagle’s medium and Ham’s F-12 (DMEM/F12; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% plasma-derived serum (PDS; First Link, Birmingham, UK) and growth factors. Porcine brain pericytes were obtained from the same fraction by seeding the microvessels onto noncoated surfaces in DMEM (Gibco, Thermo Fisher Scientific) and 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific). D3 cells (hCMEC/D3; obtained from Prof. P.-O. Couraud, INSERM, France) were maintained in rat-tail collagen coated dishes in EBM-2 medium supplemented with EGM-2MV bullet kit (Lonza, Bazel, Switzerland). Human brain vascular pericytes (HBVPs; ScienCell, Carlsbad, CA, USA) were cultured in PM medium (ScienCell) and 5% FBS.

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