Porcine brain endothelial cells (PBECs) were isolated from the grey matter of young female Vietnamese pot-bellied pigs, as described previously [37], selected with 4 µg/mL puromycin (Merck-Sigma, Darmstadt, Germany) for 4 days and cultured in Dulbecco’s modified Eagle’s medium and Ham’s F-12 (DMEM/F12; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% plasma-derived serum (PDS; First Link, Birmingham, UK) and growth factors. Porcine brain pericytes were obtained from the same fraction by seeding the microvessels onto noncoated surfaces in DMEM (Gibco, Thermo Fisher Scientific) and 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific). D3 cells (hCMEC/D3; obtained from Prof. P.-O. Couraud, INSERM, France) were maintained in rat-tail collagen coated dishes in EBM-2 medium supplemented with EGM-2MV bullet kit (Lonza, Bazel, Switzerland). Human brain vascular pericytes (HBVPs; ScienCell, Carlsbad, CA, USA) were cultured in PM medium (ScienCell) and 5% FBS.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.