The mean particle size and its distribution, and the Zeta potential of drug-loaded polymeric micelles were measured by dynamic light scattering (DLS, Nano ZS, Malvern Instruments, Malvern, UK) at 25 °C. All the samples were diluted with deionized water before measurement and then measured in triplicate.

The morphology of the drug-loaded polymeric micelles was observed using a TESCAN MAIA3 field emission scanning electron microscope (SEM, TESCAN, Brno, Czech Republic). A drop of FRET micelles without dilution was placed on a carbon-coated copper grid and air dried. The samples were stained with 2% phosphotungstic acid and observed using a transmission electron microscope (TEM, JEM-1230, JEOL, Tokyo, Japan).

X-ray powder diffraction (XRPD) was used to investigate the crystallography characteristics of the lyophilized drug-loaded polymeric micelles compared with the raw drug, the mixture of the raw drug and polymers, and blank polymeric micelles. An X-ray diffractometer (Ultima III, Rigaku, Japan) with Cu−Ka radiation was utilized, and all scans were administrated with steps of 0.02° at a rate of 4°/min over a 2θ range of 3–40°.

The interaction between the loaded drug and the polymers was investigated by 1H-NMR (400 MHz, Bruker AMX−400, Germany).

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