For the determination of the HMDA and GH contents in the substance, high-performance liquid chromatography (HPLC, Thermo Fisher Scientific, San Jose, CA, USA) was employed. The gradient method was used for both analyses.

HMDA was determined using the Luna C18(2) 250 × 4.6 mm (5 µm) column (Phenomenex, Inc., Torrance, CA, USA) at 30 °C and 264 nm. Mobile phase A is comprised of MilliQ water; mobile phase B is comprised of acetonitrile (HPLC grade). The gradient program is presented in Table 2.

Gradient Program for the HPLC analysis of HMDA.

For GH determination, the Luna C18(2) 150 × 4.6 mm (5 µm) column (Phenomenex) was used. The analysis was carried out at 25 °C and 205 nm. Mobile phase A is comprised of 0.087% sodium 1-pentanesulfonate in 1% aqueous solution of orthophosphoric acid; mobile phase B is comprised of acetonitrile (HPLC grade). The gradient program is presented in Table 3.

Gradient Program for the HPLC Analysis of GH.

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