An exploratory study of biomarkers related to the Hepatocyte growth factor/c-Met (HGF/c-Met) axis, as well as the angiogenic/inflammatory factors interleukin-6 (IL-6), interleukin-8 (IL-8), monokine induced by interferon-gamma (IFN-γ) (MIG, or CXCL9), IFN-γ-induced protein 10 (IP-10 or CXCL10) and IFN-γ-inducible T-cell alpha chemoattractant (I-TAC or CXCL11) was conducted.

Serum samples were obtained from 10 mL blood collected at baseline and 3 months after treatment initiation. Samples were aliquoted and frozen at −80 °C until use. The Luminex’s xMAP® Technology multiplex system was used to quantify simultaneously all these markers. Analysis was carried out with the Milliplex ™ MAP kit (Merck, Millipore Corporation, Kenilworth, NJ, USA), following the manufacturer-specific protocol. Concentration of each analyte was assessed according to standard calibration curves. Data are given in pg/mL.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.