An exploratory study of biomarkers related to the Hepatocyte growth factor/c-Met (HGF/c-Met) axis, as well as the angiogenic/inflammatory factors interleukin-6 (IL-6), interleukin-8 (IL-8), monokine induced by interferon-gamma (IFN-γ) (MIG, or CXCL9), IFN-γ-induced protein 10 (IP-10 or CXCL10) and IFN-γ-inducible T-cell alpha chemoattractant (I-TAC or CXCL11) was conducted.

Serum samples were obtained from 10 mL blood collected at baseline and 3 months after treatment initiation. Samples were aliquoted and frozen at −80 °C until use. The Luminex’s xMAP® Technology multiplex system was used to quantify simultaneously all these markers. Analysis was carried out with the Milliplex ™ MAP kit (Merck, Millipore Corporation, Kenilworth, NJ, USA), following the manufacturer-specific protocol. Concentration of each analyte was assessed according to standard calibration curves. Data are given in pg/mL.

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