A total number of 31 available paraffin-embedded tumor samples from primary or metastatic sites were obtained from patients included in our study. A tissue microarray (TMA) was built with 2 cores (1.5 mm diameter) per each paraffin-embedded sample. Immunohistochemistry was performed with the anti-c-Met antibody clone ab39075 at 1:100 (Abcam). Target Retrieval Solution (K8004, Dako) was used for antigen retrieval. The TMA was scanned using a Vectra Polaris slide scanner (Akoya Biosciences, Menlo Park, CA, USA) and the H-score was calculated.

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