The harvested testis were fixed in Bouin and dehydrated sequentially in 50–100% ethanol. Xylene was used to rinse the tissues for removal of dehydrant (ethanol) and then embedded in paraffin for strengthening and easy dissection. The tissues were rinsed in xylene to remove the paraffin before sectioning and washed in decreasing concentration of ethanol (100–50%) before rehydrating the tissue with water. Tissue section of 6 µm thick was made and stained with hematoxylin-eosin (H-E) dye to make it visible for photomicroscopic viewing and then observed under a light microscope at 400× magnification.

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