The 48 h-old culture of P. viridiflava was used to prepare a bacterial suspension in saline solution (0.8% NaCl). The bacterial concentration of the suspension was measured by a spectrophotometer and adjusted to an optical density of 0.1 at 530 nm, corresponding to 1 × 108 cfu/mL, and 1.5 mL of the suspension was mixed with 15 mL of NGA medium poured into the Petri dishes. After the medium solidification, two wells of 0.5 cm diameter were cut at the opposite side of the agar plates using a cork borer, and then 20 µL of the liquid or 20 µg of the solid compounds were placed into the wells. Plates with 20 µL of SDW in the wells were used as controls of bacterial growth. Three plates for each treatment were prepared. Plates were sealed with three layers of parafilm and then incubated at 27 °C for 48 h. The antibacterial activity of the tested compounds was evaluated measuring the size of the growth inhibition halo surrounding the wells.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.