Immunofluorescence staining/imaging was performed as described [34]. Briefly, sample slides were incubated with primary antibody diluted in IF buffer (0.1% BSA, 0.2% Triton-X 100, 0.05% Tween 20, 0.05% NaN3 in PBS) overnight at 4 degrees in a humidified chamber. After intensive washing (three times, 15 min each) in IF buffer, slides were incubated with fluorescence-conjugated secondary antibodies (Molecular Probes) diluted in IF buffer for 1 h at room temperature. After extensive washing in IF buffer, nuclei were counterstained with 0.5 ng/mL DAPI. After mounting with anti-fade solution, slides were visualized on Olympus IX70 microscope using CellSens software (Olympus Corp., Shinjuku City, Tokyo, Japan). Confocal fluorescence imaging for Second Harmonics Generation (SHG) technique [35] was performed on Leica Microsystems TCS SP5 multi-photon laser scanning confocal microscope using Suite Advanced Fluorescence (LAS AF) software (Leica Microsystems, Wetzlar, Germany)

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