The experiment was conducted at the animal house of the Department of Biochemistry, Adeleke University, Ede, Nigeria (University Ethical Approval Number: AUERC/FOS/01). Male Wistar albino rats of three months old, weighing between 180–200 g, were used for the study. Wistar albino rats were used for this study because their genetics, biological and behavioral characteristics are closely related to that of humans [61]. The rats were given free access to standard rat chow (Capsfeed, Osogbo, Osun State, Nigeria) and water throughout the experimental period. Experimental procedures followed were in conformity with the instructions for the care and use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996).

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