MCF10A human MECs were cultured in DMEM/F12 (Thermo Fisher, Waltham, MA, USA, Cat. # 11320033) supplemented with 5% horse serum (Thermo Fisher, Waltham, MA, USA, Cat. # 16050122); 1% penicillin/streptomycin; 10 μg/mL insulin (Sigma, St. Louis, MO, USA, Cat. # I-1882); 20 ng/mL EGF (Sigma, St. Louis, MO, USA, Cat. # E-9644); 0.5 μg/mL hydrocortisone (Sigma, St. Louis, MO, USA, Cat. # H-0888); and 100 ng/mL cholera toxin (Sigma, St. Louis, MO, USA, Cat. # C-8052) [33]. Primary human MFs were cultured in Fibroblast Medium (FM, ScienCell Research Laboratories, Carlsbad, CA, USA, Cat. #2301) supplemented with 1% Fibroblast Growth Supplement (FGS, ScienCell Research Laboratories, Carlsbad, CA, USA, Cat. #2352) and 1% penicillin/streptomycin as described [24].

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