The droplet-vitrification is a modified vitrification technique, which means that the first stages (preculture, LS and PVS treatments) are identical as described in the Vitrification method section. What sets the methods apart is the cooling and rewarming mode of the explant. In the droplet method, cooling is performed in an ultra-fast manner, where the explant trapped in a micro-drop (3–6 µL) of PVS solution is placed on aluminum sheets to be immersed directly in LN. In this process, a thermal drop of up to 1000 °C min−1 occurs. On the other hand, during rewarming, foil strips with explants are placed in a sucrose solution and left at room temperature for about 20–30 min, favoring devitrification. Thus, the damage caused by the crystallization of the solution is minimized without compromising the viability of the cells. The main interest of this method is the possibility of achieving very high rates of cooling and/or rewarming due to the low volume of cryoprotectant medium in which the explants are placed and the favorable thermal conductivity of aluminum [44,58].

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