In addition to thermal tolerance, we quantified the effects of temperature on development across populations by measuring the following: (i) hatching success, (ii) clutch size and (iii) developmental rate. To assess embryo hatching success over the incubation period, we counted the number of successfully hatched snails and compared this to the number of unsuccessful embryos using a microscope (Leica S9i, Leica Microsystems, Inc, Buffalo Grove, IL, USA). We also counted the number of initial embryos per egg case to evaluate clutch size. To measure developmental rate of embryos within egg cases, we noted the lay date of each case within 2 days of laying and checked egg cases daily for hatching. We classified an entire egg case as hatched when the first hatchling snail emerged from the opening at the top of each egg case, allowing hatchlings to crawl freely out of the egg case.

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