Peptides were evaluated for their effect upon infiltration on tobacco leaves as described previously [13]. Peptide solutions of 50, 150, and 250 µM were infiltrated (100 μL) into the mesophylls of fully expanded tobacco leaves. To test the effect of the peptide mixture, an equimolar solution was used. Six independent inoculations were carried out in a single leaf, and at least three independent inoculations were performed per peptide and concentration randomly distributed in different leaves and plants. Control infiltrations with water (negative control) or melittin (positive control) at the same molar concentration were performed. The appearance of symptoms on the leaves was followed for 48 h after infiltration and measured as a lesion diameter.

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