30S subunits from the reference strain MG 1655 were obtained by dissociation of 70S ribosomes. Briefly, 70S ribosomes purified according to [15] with minor modifications were dissociated into subunits under low magnesium concentration, fractionated in 15–30% (w/v) sucrose gradient, and the fractions containing 30S subunits were collected.

Immature ΔrbfA 30S particles from the ΔrbfA strain unable to associate with 50S subunits were purified by sucrose gradient centrifugation of the total ribosome fraction in regular salt conditions. The small ribosomal subunit fraction was considered to consist mainly of immature particles.

See Supplementary Materials for the detailed description of the procedures.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.