E. coliK-12 derivative MG-1655 (F- lambda- ilvG- rfb-50 rph-1) was used as a wild-type reference [34]. The ΔrbfA strain JW3136-1 was BW25113 (F- DE(araD-araB)567 lacZ4787(del)::rrnB-3 LAM- rph-1 DE(rhaD-rhaB)568 hsdR514 ∆rbfA::kan) was obtained from the Keio collection, a set of E. coli K-12 in-frame, single-gene knockout mutants [14].

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