Statistical analyses were performed using STATISTICA™ v8.0 (StatSoft, Tulsa, OK, USA) and MedCalc® v12.7 (MedCalc Software, Ostend, Belgium). The methylation frequency was calculated as the percentage of samples in which methylation of a particular gene was detected. Student’s t-test or Mann-Whitney U test was used to compare quantitative variables, while a 2-sided Fisher’s exact test was applied to compare categorical variables. Pearson (RP) and/or Spearman‘s (RS) rank correlation coefficients were calculated to test the associations between two variables. For BCR-free analysis, Kaplan-Meier curves with the log-rank test and Cox proportional hazards models were used. Parametric tests were applied for the analysis of TCGA data. Results were considered statistically significant when the p-value was <0.0500.

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