The antioxidant activity of the tested compounds was evaluated using a modified DPPH method. The procedure was as follows: to 2.85 mL of the DPPH radical solution (at concentrations of 0.3 mmol/dm3) dissolved in 96% (v/v) ethanol to 0.15 mL of the tested compounds (at the concentrations of 0.226 mmol/dm3) was added, and then the tube was incubated for 10 min at room temperature, followed by spectrophotometric measurements at λ = 517 nm.

The antioxidant activity of the tested samples was calculated according to the following formula:

where: %RSA—antioxidant activity, A0—mean value of the absorbance of the ethanol solution of DPPH containing 0.15 mL of the ethanol, Ap—mean value of absorbance of the ethanol solution of the DPPH radical containing 0.15 mL of the tested compound.

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