The release of EO volatiles from ICs were quantified using GC-MS by solid phase micro extraction (SPME) analysis [23,24]. The volatiles released were measured after 0, 3, 6, 9, 18, and 24 h of exposure at room temperature and 10–20% relative humidity. This was done by placing ICs on a watch glass stored in a desiccator for the allotted time. Calcium carbonate was used as a desiccant. After the allotted time had passed, 10 mg of sample was weighed into a 20 mL headspace vial (Gerstel, Linthicum, MD, US), sealed with PTFE thread tape, and capped. Five (5) mg were used for quantification of LE-1 and LE-2 due to their high amount of volatile release. Released EO volatiles were quantified using liquid standards. The SPME analysis was performed using a Scion SQ GC-MS equipped with a Gerstel multipurpose autosampler (MPS) (Gerstel, Mülheim an der Ruhr, Deutschland). The MPS allowed for automated SPME using a PDMS SPME fiber (100 μm). The SPME fiber was conditioned at 250 °C for 5 min before exposure to the sample. The samples in headspace vials were incubated for 10 min at 35 °C in the MPS agitator set at 250 rpm with an interval of 5 s on and 2 s off. The SPME fiber was exposed to the sample for 5 min with the same heating and stirring program. The SPME fiber was then desorbed in the GC inlet for 180 s before being reconditioned at 250 °C for 30 min. The same GC program described previously was used with a carrier gas flow rate of 1.00 mL/min and split delay of 1 min. LE samples were analyzed with higher initial split ratio of 1:50 to account for high volatile release. The liquid standard curve was quantified using the same GC-MS method in triplicate with 1 μL liquid injections using Gerstel MPS.

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