Extracts were diluted by half with 80 ng/μL internal standard solution (final internal standard concentration 40 ng/μL) and analyzed by GC-FID. The internal standard used for LE and LG complexes was tridecane, while octanol was used as an internal standard for GE complexes. Each extract was analyzed on a gas chromatograph (Scion 450-GC; SCION Instruments UK Ltd., Livingston, UK) equipped with a flame ionization detector (FID). The GC-FID was equipped with a Rxi®-5silms capillary column (30 m × 0.25 mm, film thickness 0.25 mm; Restek Corporation, State College, PA, US). The oven was programmed to start at 50 °C for 5 min, followed by a heating ramp of 8 °C/min to 186 °C, followed by 30 °C/min until 280 °C, where it was held for 5 min. The carrier gas was Helium at a flow rate of 1.20 mL/min. The total analysis time was 30.13 min. The detector was set at 320 °C. The injector temperature was 250 °C, 1 μL of the sample was injected manually with a split ratio of 1:20. Quantification of the EO content was done using a 400–10 ng/μL standard curve containing 40 ng/μL internal standard in hexanes. The peak from the main constituents of the EO was normalized using the internal standard peak for quantification. The mass concentration of the EO in the IC was calculated along with the % IE.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.