Extractions were done in triplicate. Ten (10) mg of IC was dissolved in 1 mL of deionized H2O. Then, 1 mL of 1:1 hexane/ethanol was added, and the solution was shaken manually. Finally, 1 mL of hexane was added, and the solutions were sonicated for 30 min at room temperature and 40 kHz in an ultrasonic bath. The organic layer was collected, and the aqueous layer was extracted twice more with 1 mL of hexane. Organic layers were combined (final volume was 3.5 mL), dried with Na2SO4, and filtered using cotton wool.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.