For analysis of vascular reactivity, maximum response, and pEC50 (negative logarithm of EC50), the values were determined from the concentration–response curves. Emax and pEC50 values were compared using Student’s t-test and a one-way analysis of variance test (one-way ANOVA), followed by the Tukey post-test. The results of the molecular experiments were analyzed by Student’s t-test or one-way ANOVA, followed by the Tukey post-test. GraphPad Prism program, version 8.0 (GraphPad Software Inc., San Diego, CA, USA) was used for data analysis. The results are expressed as mean ± standard error of the mean (SEM). The acceptable level of significance was p < 0.05.

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