RNA was isolated from endothelial cells using Trizol (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems), according to the manufacturer instructions. The mRNA levels were quantified on qPCR StepOnePlus (Life Technologies). Specific primers (TaqMan) for humans were used respectively as follows: SOD1 (Hs00533490_m1) CAT (Hs00156308_m1), Keap1 (Hs00202227_m1), Bach1 (Hs00230917_m1), and GAPDH (Hs_02786624_g1), all purchased from Life Technologies. Specific mRNA expression levels were normalized relative to GAPDH mRNA levels using the comparative 2−ΔΔCt method.

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