For treatment experiments, 250 µL mucinous tumor tissue from the PMP-2, PMCA-1 or PMCA-3 models was injected i.p, and i.p. injections of anti-angiogenic drugs, BEV (5 mg/kg) and AFL (6.25 and 12.5 mg/kg) or vehicle were initiated the following day to simulate the clinical situation after CRS with a very low remaining tumor load in the peritoneal cavity. Treatments were administered twice weekly. The drug doses used were selected based on available literature. Mice were randomly assigned to treatment groups of 6 mice. The mice were sacrificed when abdominal distention was observed, and typically, 4–5 g of mucinous tumor tissue was present when this occurred. Animals with no sign of tumor growth were sacrificed 100 days (min–max 99–106 days) after experiment initiation, which in all experiments was at least twice the median time of the survival of the vehicle treated animals. Tumor-bearing mice in these models develop abdominal distension and rarely symptoms of tumor growth. Since abdominal distension can be difficult to assess objectively, tumor growth/response was quantified by calculating a growth index as previously described [27], by combining the key parameters survival time (in days) and tumor load at the time of sacrifice (in g) to one parameter, using the equation:

where TA is the time from start of the experiment until sacrifice of the animal, and TTotal is the total duration of the experiment. One PMCA-1 control was excluded from the analysis due to no tumor take.

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