4.3. Storage and Handling of Fresh Patient and Xenograft Samples
This protocol is extracted from research article:
Anti-Angiogenic Treatment in Pseudomyxoma Peritonei—Still a Strong Preclinical Rationale
Cancers (Basel), Jun 5, 2021; DOI: 10.3390/cancers13112819

Tumor samples from patients and PDX models were aliquoted and conserved at −80 °C until analysis. Thawed samples were diluted (1:10 and 1:20) in MCDB 131 cell medium (Thermo Fisher Scientific, Waltham, MA, USA) without additions for ELISA and with 1% fetal calf serum (FCS), 100 μg/mL penicillin and 100 μg/mL streptomycin, designated “control medium” for HUVEC experiments, and the samples were passed through a syringe (18 G and 21 G) 10 times to reduce viscosity just before experiments.

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