Abstract
This method can be used to free and separate the mesophyll cells from Arabidopsis leaves. The protoplasts that are generated in this way can be used for transient expression for protein activity and subcellular localization assays.
Keywords: Protoplast, Transient expression, Arabidopsis, Transfection
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is consolidated from Jen Sheen’s protocol and Inhwan Hwang’s protocol. For references please go to the following websites for their publication lists: http://genetics.mgh.harvard.edu/sheenweb/ http://www.postech.ac.kr/center/cpit/professor.html
References
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