Abstract
This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, purified protein can be used for ligand binding assays and other biochemical experiments.
Keywords: COVID-19, SARS CoV2, Nucleocapsid protein, Recombinant expression, Protein purification
Background
Since its detection in late 2019 in Wuhan, China, SARS-CoV2 infections have been rampant around the world (Wu et al., 2020). In an effort to follow the course of early infections, the nucleocapsid protein (N) was used along with the spike protein (S) in serological assays to monitor the course of early infections in the New York area. N is one of the most highly expressed viral protein, and therefore a good target to follow. To facilitate these assays, a purification protocol was developed for N and used successfully in these studies.
Materials and Reagents
Equipment
Procedure
Data analysis
Recipes
Acknowledgments
This work was supported by the Meier & Linnartz Family Foundation. L.J. is an Investigator of the Howard Hughes Medical Institute. Competing interests: Authors declare no competing interest.
References
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