Abstract
This protocol describes a quick method for immunolocalization of meiotic proteins in maize. It includes a fixation step that allows for long-term storage of material and provides good preservation of chromatin structure.
Keywords: Immunolocalization, Meiosis, Meiocytes, Chromosomes, Maize
Background
Meiocytes are generative cells, in which specialized processes of homologous chromosomes pairing, synapsis, and recombination take place, followed by chromosome segregation to create haploid nuclei. Elucidating localization of meiotic proteins is crucial for understanding these processes. Although many variations of this protocol are possible, we found that the microwave heating step is critical for it to work reliably for immunolocalization in maize.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
Research to develop this protocol was supported by a grant from the National Science Foundation (IOS-1546792). This protocol is an adaptation of a protocol developed for Arabidopsis by Chelysheva et al. (2013).
Competing interests
Authors declare no competing interests.
References
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