Abstract
The striatum is located in the subcortical region of the forebrain, it contains medium spiny neurons, cholinergic interneurons and GABAergic interneurons, and receives dopaminergic projection in the nigrostriatal pathway. This protocol provides a method to collect synaptosomes from mouse brain dorsal stratum. The synaptosomes can be used to study dopamine uptake activity, dopaminergic terminal endocytosis/trafficking using biochemical and microscopy methods, and protein analysis (Sorkina et al., 2018).
Keywords: Striatum, Synaptosomes, Dopamine, Dopamine transporter, Endocytosis
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This work was supported by the grant DA14204 from NIH/NIDA. The author declare that there are no conflicts of interest or competing interests.
References
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