Abstract
Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones that were isolated in the screen. This is a simple protocol to extract plasmid DNA from budding yeast cultures (Robzyk and Kassir, 1992). The amount produced is small, but it is sufficient for PCR or for transformation into bacteria, where the plasmid can be amplified to provide sufficient amounts for downstream uses (e.g., restriction enzyme analysis, sequencing).
Keywords: Budding yeast, Plasmid extraction, Miniprep
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
Gal Haimovich is the recipient of the Koshland Foundation and McDonald-Leapman Grant Senior Postdoctoral Fellowships. The author declares that there are no conflicts of interest or competing interests. This protocol was adapted from Robzyk and Kassir (1992).
References
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